Induction of Callus from stem explant by using Auxins in Desmodium gangeticum (L.) DC

  1. Dr. V. N. Patil, Assistant Professor, Department of Botany, Vidyabharati College, Seloo, Dist. Wardha-442104, Maharashtra.
  2. Dr. S. S. Deokule, Professor, Department of Botany, Savitribai Phule University of Pune, Pune – 411 007, Maharashtra

Abstract:

Desmodium gangeticum (L.) DC is an important medicinal plant belongs to family Fabaceae (Leguminoceae). It is known as Salparni in Sanskrit. It is a sub-erect, under-shrub 0.6–1.2m high with irregular angled, branched woody stem. Leaves are unifoliate or trifoliate. Flowers are small, pink to purple in color. Whole plant or mainly the roots are used in medicines. It is used in Ayurvedic preparations like Dashmoola-Kwatha and Dashamoola-rishta. In the present investigation, an efficient protocol for callus induction of D. gangeticum is developed. The stem explant was inoculated with basal cut surface down on Murashige and Skoog medium supplemented with 0.25, 0.5, 0.75, 1 mg/l IAA, NAA and 2,4- D for callus induction. The better and profuse callus induction was found in MS medium supplemented with 0.5mg/l 2, 4 D from the stem explants.

Keywords: Desmodium gangeticum, Callus induction, Explants.

Download: Dr. V. N. Patil et al, Int.J.A.PS.BMS,2017,Vol.6 (1) 048-051

Leave a Reply

Your email address will not be published. Required fields are marked *